Gomez, who suffered a knock during Saturday's 0-0 draw with Stoke City at Anfield has been ruled unfit to make the trip to Rome.
However, Jordan Henderson and Trent Alexander-Arnold were both involved in full training at the Stadio Olimpico on Tuesday night and are available for the decisive encounter with Eusebio Di Francesco’s side.
Elsewhere, Sadio Mane is set to return and Alberto Moreno is also expected to shrug of a knock to put himself in contention. Long-term injury absentees Joel Matip, Alex Oxlade-Chamberlain and Emre Can remain on the sidelines.
Klopp said ahead of the training session: “Apart from the long-term injuries, only Joe is not here.
“He had a little bit of a setback in the early stages of the last game. He came through somehow, to be honest, but after the game the injury was still there so we’ve left him at home.
“It’s only a little setback for Joe, but we have to see how long he will be out for. I don’t know at the moment.
“Apart from that, I think we are pretty complete. We have 20 players plus three goalkeepers, but a lot of kids are involved and Adam Lallana is here, too.
“[Henderson and Alexander-Arnold] have had a two-day recovery and today will train normally. Alberto got a little bit of a knock. We will see, but it looks like he can train tonight. The rest should be available.”